Potential activity of GIT-27 against renal ischemia reperfusion injury: an experimental study in male rats

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Najah Hadi ¹*,Huda jabber ¹

Author Affiliations
*Corresponding Author: Electronic address: dr.najahhadi@yahoo.com. ¹Department of Pharmacology, University of Kufa.

     
Abstract

The detail mechanisms in which renal ischemia reperfusion (IRI) happens are still indistinct. Various elements required in the pathogenesis include oxidative stress, inflammation, cellular necrosis, apoptosis, renal pathophysiological changes etc. To understand the pathway of ischemia reperfusion in renal we tested the hypothesis that GIT-27 attenuated renal ischemia reperfusion injury as specific Toll-like receptor inhibitors. Adult (3 to 5 month) male Sprague Dawely rats, and their weights ranged from 180 to 390 gm, were pre-treated with Git-27 intra peritoneal, plasma NGAL and cytokines mediator’s in plasma and renal were analyzed by enzyme-linked immunosorbent assay (ELISA). And the pathological changes and cells injury in the renal were examined using Hematoxylin and Eosin staining. Improvement of renal ischemia reperfusion by GIT-27 resulted in improved renal function and greater reductions in inflammatory mediators, and kidney injury. Taken together, GIT-27 significantly improved renal function following I/R through down-regulation of Toll-like receptor and serve as a potential therapeutic in ischemia reperfusion induced acute kidney injury.

Keywords: Renal ischemia reperfusion; Git-27; NGAL

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Doi: 10.18081/2378-5225-016-12/87-99

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Research Article
Pathophysiology of Cell Injury Journal Volume 5, Issue 2, pages 87-99
Received June 22, 2016; accepted September 21, 2016; published October 29, 2016

How to cite this article
Hadi N, Jabber H. Potential activity of GIT 27 against renal reperfusion injury: an experimental study in male rats. Pathophysiology of Cell Injury Journal 2016;12:87-99.

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Article download: 17,549