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Comparison of maternal serum tumor necrosis Factor-Alpha in sever and mild pre-eclampsia versus normal pregnancy

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http://dx.doi.org/10.18081/2378-5225-016-06/1-15
Pathophysiology of Cell Injury Journal  Volume 5, Issue 1, pages 1-15
Received December 15, 2015; accepted May 02, 2016; published June 09, 2016

Azhar Mousa Al-Turiahi*

 

Abstract

Pre-eclampsia (PE) is an idiopathic disorder unique to pregnancy in human and has long been recognized as an important contributor of maternal and fetal morbidity and mortality. It is suggested that cytokines such as Tumor Necrosis Factor-alpha (TNF-α) have an important role in the pathogenesis of preeclampsia and may cause generalized endothelial dysfunction. The objective of this study is to compare the maternal serum concentration of TNF-α in severe and mild preeclampsia versus normal healthy pregnancy, and the role of this pro-inflammatory cytokine in the pathogenesis preeclampsia. This study was performed on 64 women with preeclampsia (28 mild and 36 severe preeclampsia) and 60 normotensive pregnant women at third trimester of pregnancy. All the preeclamptic cases had blood pressure ≥ 140/90 mmHg, and proteinuria ≥ 30 mg/ dl (1 + dipstick by qualitative estimation) by a random clean-catch mid-stream urine sample (which correlate with proteinuria ≥ 300 mg/24 hr. urine collection). Maternal serum TNF-α concentration was measured in all of them by Enzyme-linked immune Sorbent assay (ELISA). Mean serum TNF-α level is (=15.3571±35.80628 pg/ml) in mild PE (= 34.2222±74.21171 pg/ml) in severe PE (=22.8±52.06801 pg/ml) in normotensive pregnant women (control group). When compared with mild preeclampsia and normal pregnancy, the mean of TNF-α concentration is higher in severe preeclampsia; butthere is no statistically significant difference between preeclampsia and control group as they are compared in the view of maternal serum TNF-α concentration. These findings suggest that serum TNF-α is not significantly associated with preeclampsia; as a marker of prediction severity or pathogenesis.

Keywords: Pre-eclampsia, Serum, Tumor necrosis Factor-Alpha

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