Azhar Mousa Al-Turiahi*
Pre-eclampsia (PE) is an idiopathic disorder unique to pregnancy in human and has long been recognized as an important contributor of maternal and fetal morbidity and mortality. It is suggested that cytokines such as Tumor Necrosis Factor-alpha (TNF-α) have an important role in the pathogenesis of preeclampsia and may cause generalized endothelial dysfunction. The objective of this study is to compare the maternal serum concentration of TNF-α in severe and mild preeclampsia versus normal healthy pregnancy, and the role of this pro-inflammatory cytokine in the pathogenesis preeclampsia. This study was performed on 64 women with preeclampsia (28 mild and 36 severe preeclampsia) and 60 normotensive pregnant women at third trimester of pregnancy. All the preeclamptic cases had blood pressure ≥ 140/90 mmHg, and proteinuria ≥ 30 mg/ dl (1 + dipstick by qualitative estimation) by a random clean-catch mid-stream urine sample (which correlate with proteinuria ≥ 300 mg/24 hr. urine collection). Maternal serum TNF-α concentration was measured in all of them by Enzyme-linked immune Sorbent assay (ELISA). Mean serum TNF-α level is (=15.3571±35.80628 pg/ml) in mild PE (= 34.2222±74.21171 pg/ml) in severe PE (=22.8±52.06801 pg/ml) in normotensive pregnant women (control group). When compared with mild preeclampsia and normal pregnancy, the mean of TNF-α concentration is higher in severe preeclampsia; butthere is no statistically significant difference between preeclampsia and control group as they are compared in the view of maternal serum TNF-α concentration. These findings suggest that serum TNF-α is not significantly associated with preeclampsia; as a marker of prediction severity or pathogenesis.
Keywords: Pre-eclampsia, Serum, Tumor necrosis Factor-Alpha
1. Waugh J JS. and Smith MC, Hypertensive disorders, Edmonds DK, Dewhurst’s textbook of obstetrics and gynecology for postgraduates, 8th edition, Willey- Blackwell publishing, chapter 11, 2012;101-109.
2. Castro LC. Hypertensive disorders of pregnancy, Hacker NF, Gambone JC. and Hobel CJ. Essentials of obstetrics and gynecology, 5th edition, Elsevier publishing, chapter 14, 2010;173-182.
3. Cunningham FG. Haut JC, Leveno KJ, et al. Hypertensive disorder in pregnancy, Williams Obstetrics, 22nd edition, McGraw-Hill publishing, chapter 34, 2005; 428-437.
4. Al Ghazali B, Al-Taie AA, Hameed RJ. Study of the clinical significance of serum albumin level in Pr-eeclampsia and in the detection of its severity. American Journal of BioMedicine 2014;2(8):964-974. [Abstract/Full-Text]
5. Al Ghazali B, Aboud AF. Study the association of human chorionic gonadotrophin in prediction of preeclampsia and its severity. American Journal of BioMedicine 2014;3(8):494-503. [Abstract/Full-Text]
6. Valenzuela FJ, Perez- Sep´ulveda A, Torres M, et al. Pathogenesis of pre-eclampsia: the genetic component. Journal of Pregnancy 2012;2012:1-8. [Abstract/Full-Text]
7. Borzychowski AM, Sargent IL, Redman CW. Inflammation and pre-eclampsia. Semin Fetal Neonatal Med 2006;11(5):309-316.[PubMed]
8. Conde-Agudelo A, Althabe F, Belizan JM, Kafury-Goeta AC. Cigarette smoking during pregnancy and risk of preeclampsia: a systematic review. Am.J.Obstet.Gynecol 1999;181:1026–35. [PubMed]
9. Ahmed A, Rahman M, Zhang X, et al. Induction of placental heme oxygenase-1 is protective against TNF alpha induced cytotoxicity and promotes vessel relaxation. Mol. Med 2000;6:391-409. [PubMed]
10. Carswell EA, Old LJ, Kassel RL, Green S, Fiore N, Williamson B. An endotoxin induced serum factor that causes necrosis of tumors. Proc.Natl. Acad. Sci. U S A 1975;72:3666-3670. [PubMed]
11. Nedwin GE, Naylor SL, Sakaguchi A, et al. Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization. Nucleic Acids Res 1985;13:6361-6373. [PubMed]
12. Kriegler M, Perez C, DeFay K, Albert I, Lu SD. A novel form of TNF/cachectin is a cell surface cytotoxic transmembrane protein: ramifications for the complex physiology of TNF. Cell 1988;53:45-53. [PubMed]
13. Tang P, Hung M, Klostergaard J. Human pro-tumor necrosis factor is a homotrimer. Biochemistry 1996;35:8216–25. [PubMed]
14. Eck MJ, Sprang SR. The structure of tumor necrosis factor-alpha at 2.6 A resolution. Implications for receptor binding. J. Biol. Chem 1989;264:17595-17605. [PubMed]
15. Taylor PC, Peters AM, Pale log E, et al. Reduction of chemokine levels and leukocyte traffic to joints by tumor necrosis factor alpha blockade in patients with rheumatoid arthritis. Arthritis Rheum 2000;43:38-47.[PubMed]
16. Chen HL, Yang YP, Hu XL,Yelavarthi KK, Fishback JL, Hunt JS. Tumor necrosis factor alpha mRNA and protein are present in human placental and uterine cells at early and late stages of gestation. Am. J. Pathol. 1991;139:327-335. [PubMed]
17. Yang Y, Yelavarthi KK, Chen HL, Pace JL, Terranova PF, Hunt JS, Molecular, Biochemical, and functional characteristics of tumor necrosis factor-alpha produced by human placental cytotrophoblastic cells. J. Immunology 1993;150:5614-5624. [PubMed]
18. Vince G, Shorter S, Starkey P, et al. Localization of tumour necrosis factor production in cells at the materno-fetal interface in human pregnancy. Clin.Exp.Immunol 1992;88:174-180. [Abstract/Full-Text]
19. Hunt JS, Chen HL, Hu XL, Tabibzadeh S. Tumor necrosis factor-alpha messenger ribonucleic acid and protein in human endometrium. Biol. Reprod 1992;47:141-147. [Abstract/Full-Text]
20. Philippeaux MM, Piguet PF. Expression of tumor necrosis factor-alpha and its mRNA in the endometrial mucosa during the menstrual cycle. Am. J .Pathol 1993;143:480-486. [PubMed]
21. Jokhi PP, King A, Sharkey AM, Smith SK, Loke YW. Screening for cytokine messenger ribonucleic acids in purified human decidual lymphocyte populations by the reverse-transcriptase polymerase chain reaction. J. Immunology 1994;153:4427-4435. [PubMed]
22. Dekker G, Sibai B. Primary, secondary and tertiary prevention of preeclampsia. Lancet 2001;357:209-215. [PubMed]
23. Tabibzadeh S. Ubiquitous expression of TNF-alpha /cachectin immunoreactivity in human endometrium. Am. J. Reprod.Immunol 1991;26:1-4. [Abstract/Full-Text]
24. MacGillivray I, Rose GA. Rowe B. Blood pressure survey in pregnancy. Clin. Sci 1969;37:395-407.
25. King A, Jokhi PP, Smith SK, Sharkey AM, Loke YW. Screening for cytokine mRNA in human villous and extra villous trophoblasts using the reverse transcriptase polymerase chain reaction (RT-PCR). Cytokine 1995;7:364-371. [PubMed]
26. Hung TH, Charnock-Jones DS, Skepper JN, and Burton GJ. Secretion of tumor necrosis factor-alpha from human placental tissues induced by hypoxia reoxygenation causes endothelial cell activation in vitro: a potential mediator of the inflammatory response in preeclampsia. Am. J. Pathol 2004;164:1049-1061. [PubMed]
27. Pijnenborg R, McLaughlin PJ, Vercruysse L, et al. Immunolocalization of tumour necrosis factor-alpha (TNF alpha) In the placental bed of normotensive and hypertensive human pregnancies. Placenta 1998;19:231-239. [PubMed]
28. Yelavarthi KK, Hunt JS. Analysis of p60 and p80 tumor necrosis factor-alpha receptor messenger RNA and protein in human placentas. Am. J. Pathol 1993;143:1131-1141. [PubMed]
29. Daher S, Fonseca F, Ribeiro O, Musatti C, Gerbase-Delima M. Tumor necrosis factor during pregnancy and at the onset of labor and spontaneous abortion. European Journal of Obstetrics Gynecology and Reproductive Biology 1999;83:77-79. [Abstract/Full-Text]
30. Lewis D, Canzoneri B, Wang Y. Maternal circulating TNF-α levels are highly correlated with IL-10 levels, but not IL-6 and IL-8 levels, in women with pre-eclampsia. American Journal of Reproductive Immunology 2009;62:269-274. [Abstract/Full-Text]
31. Hauth J, Ewell M, Levine R, et al. Pregnancy outcomes in healthy nulliparas who developed hypertension. Obstetrics and Gynecology 2000;95:24-28. [PubMed]
32. Haider S. Knὃfler M. Human Tumor Necrosis Factor. Physiological and pathological roles in placenta and endometrium. Placenta 2009;30:111-123. [PubMed]
33. Jonsson Y, Ruber M, Matthiesen L, et al. Cytokine mapping of sera from women with preeclampsia and normal pregnancies. J. Reprod. Immunology 2006;70:83–91. [PubMed]
34. Lok C, Boing A, Reitsma P, et al. Expression of inflammation-related genes in endothelial cells is not directly affected by microparticles from preeclamptic patients. J. Lab. Clin. Med 2006;147:310–320. [PubMed]
35. Afshari JT, Ghomian N, Shameli A, et al. Determination of interlukin-6 and tumor necrosis factor-alpha concentrations in Iranian-Khorasanian patients with preeclampsia. BMC Pregnancy and Childbirth 2005;5:14. [Abstract/Full-Text]
36. Roudsari FV, Ayati S, Ayatollahi H, et al. Comparison of maternal serum Tumor Necrosis Factor-alpha (TNF-α) in severe and mild preeclampsia versus normal pregnancy. Iranian Journal of Reproductive Medicine 2009;7(4):153-156. [Abstract/Full-Text]
37. Kazamierczak IA, Korzeniewski J, Grybosm. Evaluation of congenital risk on the basic of cytokines IL-1 beta, IL-6 and TNF alpha levels in blood serum and amniotic liquid in pregnant women and in umbilical blood of neonates. Ginekol Pol 2003;74(4):297-302. [Abstract/Full-Text]
38. Solomon C, Seely E. Hypertension in pregnancy a manifestation of the insulin resistance syndrome? Hypertension 2001;37:232-239. [Abstract/Full-Text]
39. Muzammil S, Singhal U, Gulati R, Bano I. Serum tumor necrosis factor-alpha in pre eclampsia. Indian. J. Physiol. Pharmacology 2005;49:236-240. [Abstract/Full-Text]
40. Mihu D, Costin N, Blaga LD, Ciuchina S, Pop RB. Implication of Tumor Necrosis Factor – Alpha in Preeclampsia. Applied Medical Informatics 2008;23:11-18. [Abstract/Full-Text]
41. Vitoratos N, Economou E, Iavazzo C, Panoulis K, Creatsas G. Maternal Serum levels of TNF-Alpha and IL-6 Long after Delivery in preeclamptic and Normotensive Pregnant Women. Mediators of Inflammation. Clinical Study 2010:908649. [Abstract/Full-Text]
42. Founds SA, Powers RW, Patrick TE, et al. A Comparison of Circulating TNF-α in Obese and Lean Women with and without Preeclampsia. Hypertension in Pregnancy 2008;27:39-48. [PubMed]
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